Phagocytosis-induced modulation of human neutrophil chemotaxis receptors.

We investigated the effect of a major physiologic event, phagocytosis. on the ability of human neutrophils (PMN) to bind the synthetic chemotactic peptide, 3H-formyl-methionyl-leucyl-phenylalanine (3H-FMLP). We found that both latex and opsonized zymosan (OPZ) particles caused dosedependent suppression of 3H-FMLP binding and abolition of 3H-FMLP binding with concentrations of OPZ greater than 2 mg/mI. This ‘particle effect was completely inhibited by incubation of PMN with 1 0 mM 2-deoxyglucose before. but not after. phagocytosis. The particle effect was partially inhibited (28%) by anaerobic conditions and CGD neutrophils. but not by superoxide dismutase. catalase. or oxygen radical scavengers. In addition. supernatants from PMN that had been incubated with OPZ caused 55% reduction of 3H-FMLP binding by fresh PMN. The effect of PMN-OPZ supernatants was inhibited by 1 mM phenyl-